Syllabus | PHARMACEUTICAL MICROBIOLOGY (Theory) | B. Pharmacy

Course: B Pharmacy 3rd semester /2nd Year

Name of Subject: PHARMACEUTICAL MICROBIOLOGY (Theory)

Subject Code: BP 303 T

S. No. Contents of the Topics             Domain Time(Hrs)
1. Introduction, History of microbiology
Its  branches,
Scope and its importance.
Introduction to Prokaryotes and Eukaryotes
Study  of  ultra-structure  and  morphological  classification  of  bacteria,
Nutritional requirements,
raw materials used for culture media
Physical parameters for growth,
Growth curve,
Isolation and preservation methods for pure cultures, Cultivation of anaerobes
Quantitative measurement of bacterial growth (total & viablecount).
Study  of  different  types  of  phase  contrast  microscopy,  dark  field microscopy and electron microscopy.
 

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2 Identification of bacteria using staining techniques (simple, Gram’s &Acid fast staining) and biochemical tests (IMViC).
Study of principle, procedure, merits, demerits and applications of Physical, chemical and mechanical method of sterilization.
Evaluation of the efficiency of sterilization methods.
Equipments employed in large scale sterilization. Sterility indicators

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3 Study   of   morphology, classification,   reproduction/replication   and cultivation of Fungi and Virus.

Classification and mode of action of disinfectants.
Factors influencing disinfection, antiseptics and their evaluation.  For bacteriostatic and bactericidal actions Evaluation of bactericidal & Bacteriostatic. Sterility testing of products (solids, liquids, ophthalmic and other sterile products) according to IP, BP and USP.

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4 Designing of  aseptic  area,  laminar  flow  equipments;  study of  different sources of contamination in an aseptic area and methods of prevention, clean area classification.
Principles and methods of different microbiological assay.
Methods for standardization of antibiotics, vitamins and amino acids.

Assessment of a new antibiotic and testing of antimicrobial activity of a new substance.

General aspects-environmental cleanliness.

Must Know

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5 Types   of   spoilage
factors   affecting   the   microbial   spoilage   of pharmaceutical products, sources
types  of  microbial  contaminants,
assessment of microbial contamination and spoilage.

Preservation of pharmaceutical products  using  antimicrobial  agents, evaluation of microbial stability offormulations.

Growth  of  animal  cells  in  culture,  general  procedure  for  cell  culture, Primary, established and transformed cell cultures.

Application of cell cultures in pharmaceutical industry and research.

 

 

 

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