Syllabus | Instrumental Methods of Analysis (Theory) | B. Pharmacy

Course: B. Pharmacy Semester- 7th / 4th year
Name of Subject: Instrumental Methods of Analysis (Theory)
Subject Code: BP701T
S. No. Contents of the Topics             Domain Time(Hrs)
1. UV Visible spectroscopy

Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra,

Beer and Lambert’s law, Derivation and deviations.

Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications – Spectrophotometric titrations, Single component and multi component analysis

Fluorimetry

Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence,

Quenching, instrumentation and applications

 

 

Nice to Know

 

Must Know

Nice to Know

 

 

 

Must Know

 

 

 

 

 

 

10

2 IR spectroscopy

Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations

Instrumentation – Sources of radiation, wavelength selectors, detectors – Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications

Flame Photometry-Principle, interferences, instrumentation and applications

 Atomic absorption spectroscopy– Principle, interferences, instrumentation and applications

Nepheloturbidometry– Principle, instrumentation and applications

 

Nice to Know

 

Must Know

 

 

Must Know

10
3 Introduction to chromatography

 

Adsorption  and  partition  column  chromatography-Methodology,  advantages,

Disadvantages and applications.

Thin  layer  chromatography-  Introduction,  Principle,  Methodology,  Rf  values,

Advantages, disadvantages and applications.

Paper         chromatography-Introduction, methodology,   development   techniques, advantages, disadvantages and applications

Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques

of paper, gel, capillary electrophoresis, applications

Nice to know

 

 

Must Know

 

 
 

Must Know

10
4 Gas  chromatography  –  Introduction,  theory,  instrumentation,  derivatization,

temperature programming, advantages, disadvantages and applications

High        performance   liquid   chromatography   (HPLC)-Introduction,   theory,

Instrumentation, advantages and applications.

 

 

Must know

8
5 Ion exchange chromatography- Introduction, classification, ion exchange resins,

properties,  mechanism  of  ion  exchange  process,  factors  affecting  ion  exchange,

methodology and applications

Gel chromatography- Introduction, theory, instrumentation and applications

Affinity chromatography- Introduction, theory, instrumentation and applications

Must know

 

Nice to know

 

Nice to know

 

 

7

Admission 2017