Syllabus | ADVANCED INSTRUMENTATION TECHNIQUES | B. Pharmacy

 

Course: B Pharmacy 4th Year / 8th Semester

 

Name of Subject: ADVANCED INSTRUMENTATION TECHNIQUES

 

Subject Code: BP 811 ET.

Sr. No.

Topic (Experiments)

Domain

Time
(Hrs)

Nuclear Magnetic Resonance spectroscopy

Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin – spin coupling, relaxation, instrumentation and applications
Mass  Spectrometry-  Principles,  Fragmentation,  Ionization  techniques  –

Electron  impact,  chemical  ionization,   MALDI,  FAB,             Analyzers-Time  of
flight and Quadrupole, instrumentation, applications

Must to know 10
Thermal Methods of Analysis: Principles, instrumentation and applications
of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)
X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X-ray
Crystallography, rotating crystal technique, single crystal diffraction ,powder
diffraction, structural elucidation and applications.
Desirable to know 10
Calibration and validation-as per ICH and USFDA guidelines
Calibration of following Instruments
Electronic  balance,  UV-Visible  spectrophotometer,  IR  spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC
  10
Radio immune assay: Importance, various components, Principle, different

methods, Limitation and Applications of Radio immuno assay

Extraction techniques:General principle and procedure involved in the solid

phase extraction and liquid-liquid extraction

Must to know 8
Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS. Nice to know 7