Curriculum | Physical Pharmaceutics-II (Theory) | B. Pharmacy

Course: B Pharmacy 3rd semester /2nd Year

Name of Subject: PHARMACEUTICAL MICROBIOLOGY (Theory)

Subject Code: BP 303 T

Unit

Topic (Contents)

Learning Objective ( BY the end of lesson should be able to)

Teaching Guidelines

Methodology

Time (Hrs)

1

Introduction, history of microbiology,  its  branches,  scope  and  its importance.
Introduction to Prokaryotes and Eukaryotes Study  of  ultra-structure  and  morphological  classification  of  bacteria, nutritional requirements, raw materials used for culture media and physical parameters for growth, growth curve, isolation and preservation methods for pure cultures, cultivation of anaerobes, quantitative measurement of bacterial growth (total & viable count).
Study  of  different  types  of  phase  contrast  microscopy,  dark  field microscopy and electron microscopy.
At the end of the lecture student should be able to describe and demonstrate historical background of of  microbiology.

Demonstrate about the different culture media and different types of microscopes.

  • Introduction to microbiology
  • Contribution of various scientists
  • Scope
  • Classification of Microbes
  • Raw materials used for culture media and physical parameters for growth,
  •  growth curve, isolation and preservation methods for pure cultures,
  • cultivation of anaerobes,
  • quantitative measurement of bacterial growth (total & viable count).
  • Microbial culture techniques
  • Application of Microscopy in microbiology
Instructive

Power point presentation

10

2

Identification of bacteria using staining techniques (simple, Gram’s &Acid fast staining) and biochemical tests (IMViC).
Study of principle, procedure, merits, demerits and applications of Physical, chemical and mechanical method of sterilization.
Evaluation of the efficiency of sterilization methods.
Equipments employed in large scale sterilization. Sterility indicators
In the end of the lecture student should be able to describe and demonstrate about the staning techniques and sterlization methods. Identification of bacteria using staining techniques

  • Simple
  • Gram’s  Staining
  • Acid fast staining

Biochemical tests
Sterilization

  • Physical
  • Chemical
  • Mechanical

Evaluation of the efficiency of sterilization methods.
Equipments

  • Autoclave
  • Hot Air Oven

Sterility Indicators

—Instructive
—Power point presentation
—Tutorials

10

3

Study   of   morphology,   classification,   reproduction/replication   and cultivation of Fungi and Virus.

Classification and mode of action of disinfectants.
Factors influencing disinfection, antiseptics and their evaluation.  For bacteriostatic and bactericidal actions Evaluation of bactericidal & Bacteriostatic. Sterility testing of products (solids, liquids, ophthalmic and other sterile products) according to IP, BP and USP.

In the end of the lecture student should be able to describe and demonstrate in detail about Sterilization and disinfection techniques Fungi and Virus

  • Morphology
  • Classification
  • Reproduction

Disinfectants

  • Classification
  • Mode of Action
  • Factors Affecting Disinfection

Antiseptics and their evaluation
Sterility Testing

  • Membrane Filteration Method
  • Direct Inoculation Method

Sterility testing of products (solids, liquids, ophthalmic and other sterile products

–Instructive

–Power point presentation
–Tutorials

10

4

Designing of  aseptic  area,  laminar  flow  equipments;  study of  different sources of contamination in an aseptic area and methods of prevention, clean area classification.
Principles and methods of different microbiological assay. Methods for standardization of antibiotics, vitamins and amino acids.
Assessment of a new antibiotic and testing of antimicrobial activity of a new substance.
General aspects-environmental cleanliness.
In the end of the lecture student should be able to describe in detail about the aseptic conditions, different types of microbiological assay, activity of antibiotics and standardization of antibiotics. Aseptic Area
Laminar Air Flow
Different sources of contamination

  • Atmosphere
  • Operator
  • Raw Material
  • Equipments

Methods of prevention
Microbiological Assay
Methods

  • The cylinder-plate (or cup-plate) method
  • The turbidimetric (or tube assay) method.

Methods for standardization of antibiotics, vitamins and amino acids.

  • Agar Diffusion Method

Assessment of a new antibiotic and testing of antimicrobial activity of a new substance.

Environmental cleanliness.

—Instructive

—Power point presentation
—Tutorials

8

5

Types   of   spoilage,   factors   affecting   the   microbial   spoilage   of pharmaceutical products, sources  and  types  of  microbial  contaminants, assessment of microbial contamination and spoilage.
Preservation of pharmaceutical  products  using  antimicrobial  agents, evaluation of microbial stability of formulations.
Growth  of  animal  cells  in  culture,  general  procedure  for  cell  culture, Primary, established and transformed cell cultures.
Application of cell cultures in pharmaceutical industry and research.
In the end of the lecture student should be able to describe and demonstrate in detail about the microbial contaminations, preservation of pharmaaceutical products and microbial stability of formulations. Application of cell culture in pharma industries.
  • Types   of   spoilage,
  • Factors   affecting   the   microbial   spoilage   of pharmaceutical products,
  • Sources  and  types  of  microbial  contaminants, assessment of microbial contamination and spoilage.
  • Preservation of pharmaceutical  products  using  antimicrobial  agents, evaluation of microbial stability of formulations.
  • Growth  of  animal  cells  in  culture
  • General  procedure  for  cell  culture, Primary, established and transformed cell cultures.
  • Application of cell cultures in pharmaceutical industry and research.
Instructive

Tutorials
Case Presentation

7

Admission 2017