Curriculum | PHARMACEUTICAL MICROBIOLOGY (Practical) | B. Pharmacy

Course: B. Pharmacy 3rd Semester/ 2nd Year

Name of Subject: PHARMACEUTICAL MICROBIOLOGY (Practical)

Subject Code: BP 307P

Unit

Topic (Contents)

Learning Objective ( BY the end of lesson should be able to)

Teaching Guidelines

Methodology

Time (Hrs)

1

Introduction and study of different equipments and processing, e.g., B.O.D. incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator, microscopes used in experimental microbiology. At the end of the practical student should be able to describe and demonstrate different types of equipments used in microbiology laboratory To Cover parts and Functioning of B.O.D. incubator, laminar flow, aseptic hood, autoclave, hot air sterilizer, deep freezer, refrigerator, microscopes used in experimental microbiology Instructive
Actual practical performing by the students in laboratory and assisting the students to perform the practical.

4

2

 Sterilization of glassware, preparation and sterilization of media. In the end of the practical student should be able to describe and perform sterlization of glassware and preparation of different media. To cover Nutritional requirements to prepare media and sterilization procedure to sterilize glasswares Instructive

Actual practical performing by the students in laboratory and assisting the students to perform the practical.

4

3

Sub culturing of bacteria and fungus. Nutrient stabs and slants preparations. In the end of the practical student should be able to describe and demonstrate bacteria and fungi culturing. To cover subculturing procedure and slants preparations Instructive

Actual practical performing by the students in laboratory and assisting the students to perform the practical.

4

4

Staining methods- Simple, Grams staining and acid fast staining (Demonstration with practical). In the end of the practical student should be able to describe and demonstrate different types of staining methods. To cover
Simple staining, Grams staining and acid fast staining with procedure and requirements
Instructive

Actual practical performing by the students in laboratory and assisting the students to perform the practical.

4

5

Isolation of pure culture of micro-organisms by multiple streak plate technique and other techniques. In the end of the practical student should be able to describe and demonstrate different isolation of pure culture of microorganisms. To cover what is multiple streaking and detailed procedure of isolation of pure culture by different techniques Instructive

Actual practical performing by the students in laboratory and assisting the students to perform the practical.

4

6

Microbiological assay of antibiotics by cup plate method and other methods In the end of the practical student should be able to describe and demonstrate activity of antibiotics using cup plate agar method. To cover requirements and Procedure of cup plate method and direct inoculation method Instructive

Actual practical performing by the students in laboratory and assisting the students to perform the practical.

4

7

Motility determination by Hanging drop method. In the end of the practical student should be able to describe and demonstrate different types of motility of microorganisms. Requirements and Procedure of Hamging drop method Instructive

Actual practical performing by the students in laboratory and assisting the students to perform the practical.

4

8

Sterility testing of pharmaceuticals. In the end of the practical student should be able to describe and demonstrate sterlity of different pharmaceutical formulations. What is sterility tesing, their types as well as procedures Instructive

Actual practical performing by the students in laboratory and assisting the students to perform the practical.

4

9

Bacteriological analysis of water In the end of the practical student should be able to describe bacteriological assay of water. Requirement and procedure of Bacteriological analysis of water Instructive
Actual practical performing by the students in laboratory and assisting the students to perform the practical.

4

10

Biochemical test (IMViC reactions) In the end of the practical student should be able to describe and demonstrate biochemical test. Requirement and procedure of Biochemical tests Instructive
Actual practical performing by the students in laboratory and assisting the students to perform the practical.

4

Admission 2017