Curriculum | Pharmaceutical Biotechnology (Theory) | B. Pharmacy

 

Course: B Pharmacy 6th Semester /3rd Year,

 

 

Name of the Subject: Pharmaceutical Biotechnology (Theory)

 

 

Subject Code: BP605T

 

Unit

Subunit

Topic (Contents)

Learning Objective

Teaching Guidelines

Methodology

Time (Hrs)

1 1.1 Brief introduction to Biotechnology with reference to Pharmaceutical Sciences. ( BY the end of lesson student should be able to)

describe the importance and scope of biotechnology.

  • Concept of Biotecnology
  • Role of biotechnology in pharmacy
  • Scope
Instructive and student interactive session.
Video tutorials.

1

  1.2 Enzyme Biotechnology- Methods of enzyme immobilization and applications. Explain the immobilization of enzymes.
  • Enzyme
  • Role of enzyme in drug delivery
  • Immobilization
  • Applications
Video tutorials.
Instructive and student interactive session.

2

  1.3 Biosensors- Working and applications of biosensors in Pharmaceutical Industries. Describe the construction and working of biosensors.
  • Biosensors
  • Role of Biosensors in drug delivery
  • Applications
Power point presentation and video tutorials
Instructive and student interactive session.

2

  1.4 Brief introduction to Protein Engineering. Explain protein engineering.
  • Protein
  • Protein Synthesis
  • Bioengineering
Instructive
Power point presentation and video tutorials
Instructive and student interactive session.

1

  1.5 Use of microbes in industry. Production of Enzymes- General consideration – Amylase, Catalase, Peroxidase, Lipase, Protease, Penicillinase. Describe the industrial importance of microbes.
  • Industrial Microbiology
  • Microorganisms
  • Role of microorganisms in drug delivery
  • Enzyme catabolisms
PowerPoint presentations. video tutorials
Instructive and student interactive session.

2

  1.6 Basic principles of genetic engineering Explain genetic engineering.
  • Genes
  • Gene Delivery
  • Genetic engineering
Instructive and video tutorials, Power point presentation
and student interactive session.

2

2 2.1 Study of cloning vectors, restriction endonucleases and DNA ligase.

 

describe the concept of cloning.
  • Vectors,
  • Cloning
  • Ligase
  • ER
Instructive and video tutorials.
Power point presentation
and student interactive session

2

  2.2 Recombinant DNA technology. Application of genetic engineering in medicine. Explain Recombinanat DNA tech. Applications.
  • DNA
  • RNA
  • Recombinant Technology
Instructive and video tutorials
Power point presentation
and student interactive session

2

  2.3 Application of r DNA technology and genetic engineering in the products:
Interferon b) Vaccines- hepatitis- B c) Hormones- Insulin
Describe the concept of vaccine, interferons and harmones.
  • Vaccines
  • Interferon’s
  • Genetic engineering
  • Hormones
PowerPoint presentations.
student interactive session
video tutorials

2

  2.4 Brief introduction to PCR Explain PCR
  • Concept of PCR
  • Applications of PCR
Demonstration.
PowerPoint presentations.
student interactive session
video tutorials

2

  2.5 Types of immunity-humoral immunity, cellular immunity. describe the mechanism of immunity.
  • Concept of immunity
  • Role of Immunity in Human Being
  • Types of Immunity
Instructive and video tutorials
Demonstration.
PowerPoint presentations.

2

3 3.1 Structure of Immunoglobulins,  Structure and Function of MHC Explain the concept of immunoglobulins.
  • Antigens
  • Antiboldies
  • Immunoglobulins
  • Structure and Function of MHC

 

Instructive and video tutorials.
Demonstration.
PowerPoint presentations.

2

  3.2 Hypersensitivity reactions, Immune stimulation and Immune suppressions. describe the concept of immune suppression and stimulation.
  • Hypersensitivity reactions,
  • Immune stimulation
  • Immune suppressions.
PowerPoint presentations.
Instructive and video tutorials.
Demonstration.

2

  3.3 General method of the preparation of bacterial vaccines, toxoids, viral vaccine, antitoxins, serum-immune blood derivatives and other products relative to immunity. Explain the procedure for the production of vaccines and related compounds.
  • Method of the preparation of bacterial vaccines,
  • Toxoids,
  • Viral vaccine,
  • Antitoxins,
  • Serum-immune blood derivatives
  • Other products relative to immunity.
Instructive and student interactive session.
video tutorials.
Demonstration.

2

  3.4 Storage conditions and stability of official vaccines Describe the storage and stability of vaccines.
  • Storage conditions and stability of official vaccines
Instructive and student interactive session.

2

  3.5 Hybridoma technology- Production, Purification and Applications Explain the hybridoma technology and its applications.
  • Hybridoma technology-
  • Production,
  • Purification
  • Applications
Powerpoint point presentation.

2

4 4.1 Immuno blotting techniques- ELISA, Western blotting, Southern blotting. Describe the immune blotting techniques.
  • Immuno blotting techniques-
  • ELISA,
  • Western blotting,
  • Southern blotting.
Instructive, Demonmtration. Didactic lecture through power point projection.

2

  4.2 Genetic organization of Eukaryotes and Prokaryotes Explain prokaryotes and eucaryotes.
  • Genetic organization of Eukaryotes and Prokaryotes
Instructive, Demonstration.
PowerPoint presentations.

1

  4.3 Microbial genetics including transformation, transduction, conjugation, plasmids and transposons. describe the concept of transformation and transduction.
  • Microbial genetics including transformation,
  • Ransduction,
  • Conjugation,
  • Plasmids
  • Transposons.
Didactic lecture through power point projection. Instructive, Demonmtration.

2

  4.4 Introduction to Microbial biotransformation and applications. Enumerate the applications of microbial biotransformation
  • Introduction to Microbial biotransformation and applications.
Didactic lecture through power point projection. Instructive, Demonmtration.

2

  4.5 Mutation. describe d the process of mutation.
  • Mutation.
Instructive. Didactic lecture through power point projection.

1

5 5.1 Types of mutation/mutants Explain mutation types.
  • Types of mutation/mutants
Instructive. Didactic lecture through power point projection.

1

  5.2 Fermentation methods and general requirements, study of media, equipments, sterilization methods, aeration process, stirring. Describe the concept of fermentation.
  • Fermentation methods
  • Requirements of media,
  • equipments,
  • sterilization methods,
  • aeration process, stirring.
Didactic lecture through power point projection and video tutorials.

2

  5.3 Large scale production fermenter design and its various controls. Explain the construction and working of various fermenters.
  • Large scale production fermenter design and its various controls.
Video tutorials, Didactic lecture through power point projection and video tutorials.

2

  5.4 Study of the production of – penicillins, citric acid, Vitamin B12, Glutamic acid, Griseofulvin, Describe the process of production of antibiotics.
  • Penicillins,
  • Citric acid,
  • Vitamin B12,
  • Glutamic acid,
  • Griseofulvin,
Didactic lecture through power point projection and video tutorials.

2