Curriculum | Instrumental Methods of Analysis (Theory) | B. Pharmacy

Course: B. Pharmacy Semester- 7th / 4th year

Name of Subject: Instrumental Methods of Analysis (Theory)

Subject Code: BP701T

Unit

Topic (Contents)

Learning Objective ( BY the end of lesson should be able to)

Teaching Guidelines

Methodology

Time (Hrs)

1

UV Visible spectroscopy
Electronic transitions, chromophores, auxochromes, spectral shifts, solvent effect on absorption spectra, Beer and Lambert’s law, Derivation and deviations.

Instrumentation – Sources of radiation, wavelength selectors, sample cells, detectors-Photo tube, Photomultiplier tube, Photo voltaic cell, Silicon Photodiode.

Applications – Spectrophotometric titrations, Single component and multi component analysis

 

 

Fluorimetry

Theory, Concepts of singlet, doublet and triplet electronic states, internal and external conversions, factors affecting fluorescence, quenching, instrumentation and applications

 

Explain  what is spectra,spectroscopy and spectrometry

Comprehend the interaction of matter with electromagnetic radiations and its applications in drug analysis

Describe instruments components ,principal and uses

Explain applications of uv visible spectrophotometer

 

 

 

 

 

 

 

 

 

 

 

  • Explain  theory, principle, instrumentation of Fluorimetry and its applications in drug analysis
To Cover Electronic transitions, chromophores, auxochromes,
Spectral shifts:

  • Bathochromic
  • Hypsochromic
  • Hyperchromic
  • Hypochromic

Solvent effect on absorption spectra

Beer and Lambert’s law, Derivation and deviations

Instrumentation
Sources
of radiation, wavelength selectors,
sample cells, Detectors

  • Photo tube,
  • Photomultiplier tube
  • Photo voltaic cell,
  • Silicon Photodiode.

Applications – Spectrophotometric titrations, Single component and multi component analysis

Fluorimetry
Theory
Principle,
Concepts of singlet, doublet and triplet electronic states, internal and external conversions

Factors affecting Fluorescence

  • Light Intensity
  • Conc.
  • Nature of  sample etc.

Instrumentation

  • Source of Flame
  • Sample cell
  • Detectors

Applications

Instructive

Video presentation

workshop

 

Power point presentations

10

2

IR spectroscopy

Introduction, fundamental modes of vibrations in poly atomic molecules, sample handling, factors affecting vibrations

Instrumentation – Sources of radiation, wavelength selectors, detectors – Golay cell, Bolometer, Thermocouple, Thermister, Pyroelectric detector and applications

Flame Photometry-Principle, interferences, instrumentation and applications

 

 

 

 

Atomic absorption spectroscopy– Principle, interferences, instrumentation and applications

Nepheloturbidometry– Principle, instrumentation and applications

Explain the concept ,principal, types of modes ,vibrations  and various factors affecting vibrations of IR Spectroscopy
explain applications of IR  spectroscopy

 

 

 

 

 

 

 

Describe flame photometer and their instrumentation and  application in pharmaceuticals industry

 

 

 

Know about AAS ,Principal ,instrumentation and its application in pharmacy

 

Explain Nepheloturbidometry Principal ,instrumentation and its application in pharmacy

IR spectroscopy
Introduction,
Fundamental modes of vibration

  • Bending
  • Twisting
  • Wagging
  • Rocking

Samplehandling,
factors affecting vibrations

  • Polarity of solvent
  • Dipole moment
  • Geometry
  • Light intensity
  • Nature of sample

Instrumentation

  • Sources of radiation
  • wavelength selectors
  • Detectors –
  • Golay cell,
  • Bolomete
  • Thermocouple,
  • Thermiste
  • Pyroelectric detector

Applications

 

Atomic absorption spectroscopy

  • Principle,
  • interferences,
  • instrumentation
  •  applications

 

Nepheloturbidometry

  • Principle
  • Instrumentation
  • Source of flame
  • Sample injector
  • Compressor
  • Collector

Applications

 

Instructive

Power point presentations

 

Video presentation

Group discussion with research paper

 

10

3

Introduction to chromatography

Adsorption  and  partition  column  chromatography-Methodology,  advantages,
Disadvantages and applications.

 

Thin  layer  chromatography-  Introduction,  Principle,  Methodology,  Rf  values,
Advantages, disadvantages and applications.

 

 

 

Paper        chromatography-Introduction,   methodology,   development   techniques,
advantages, disadvantages and applications

 

 

Electrophoresis– Introduction, factors affecting electrophoretic mobility, Techniques
of paper, gel, capillary electrophoresis, applications

explain and Discuss  the concept of chromatography ,types of chromatography ,methodology and various merits and demerits with example and its application

 

explain TLC, basic principle and concept of Rf value and its application

 

Comprehend the  theory, principle, development techniques ,separation and analysis of drugs methodology and various merits and demerits  of paper chromatography and its application in pharmacy

 

 

Explain theory of  electrophoresis ,factors affecting electrophoretic mobility and its application

 

Chromatography

  • Introduction
  • Types

Adsorption Chromatography

  • Principle
  • Materials
  • Applications

Partition Chromatography

  • Principle
  • Solvent system
  • Materials
  • Applications

Thin Layer Chromatography

  • Principle
  • Methodology
  • Rf values
  • Advantages
  • Disadvantages
  • Applications

Paper chromatography

  • Introduction
  • Types
  • Principle
  • Technique
  • Advantages
  • Disadvantages
  • Applications

Electrophoresis

  • Introduction
  • Factors affecting electrophoretic mobility,
  • Techniques of paper, gel,
  • Capillary electrophoresis, Applications
 

Instructive

 

Video presentation

 

 

Power point presentations

 

 

 

 

10

4

Gas  chromatography  –  Introduction,  theory,  instrumentation,  derivatization,
temperature programming, advantages, disadvantages and applications

 

High        performance   liquid   chromatography   (HPLC)-Introduction,   theory,
Instrumentation, advantages and applications.

Discuss what is gas chromatography ,theory ,instrumentation ,applications ,basic components of the instruments or its needs

 

 

Explain HPLC ,DETECTORS, Working of software, application, drugs  separation analysis, merits and demerits

Perform quantitative & qualitative analysis of drugs using various analytical instruments

Gas  chromatography 

  • Introduction
  • Theory
  • Instrumentation
  • Carrier Gas
  • Injection Port
  • Column
  • Detector
  • Derivatization
  • Advantages
  • Disadvantages
  • Applications

HPLC

  • Principle
  • Solvent system
  • Pump/Pressure
  • Types of column
  • Types of injector
  • Types of detector
  • Data recorder
  • Software
  • Advantages and applications
Instructive with flow chart

Video presentation

Workshop

Power point presentations

8

5

Ion exchange chromatography- Introduction, classification, ion exchange resins,
properties,  mechanism  of  ion  exchange  process,  factors  affecting  ion  exchange,
methodology and applications

Gel chromatography- Introduction, theory, instrumentation and applications

 

 

 

 

Affinity chromatography- Introduction, theory, instrumentation and applications

 

Know about what is ion exchange  chromatography ,theory ,methodology ,applications

 

 

Explain what is gel chromatography ,theory ,instrumentation and its applications

 

 

 

 

Discuss what is affinity  chromatography ,theory ,instrumentation and its applications

Ion exchange chromatography-

  • Introduction
  • Principle
  • Ion exchange resins
  • Classification
  • Mechanism of Ion exchange process
  • Factors  affecting  ion  exchange,
  • Methodology and Applications

Gel chromatography-

  • Introduction
  • Theory
  • Instrumentation Applications

Affinity chromatography-

  • Introduction
  • Theory,
  • Instrumentation
  • Applications

 

 

Power point presentations

Video presentation

 

 

 

 

7

Admission 2017