Curriculum | Instrumental methods of analysis (Practical) | B. Pharmacy

Course: B. Pharmacy VIIth Semester/ 4th Year
Name of Subject: Instrumental methods of analysis (Practical)
Subject Code:  BP705 P
Sr. No. Contents of the Topic Learning objectives Teaching Guidelines Methodology Time

(Hrs)

1.       Determination of absorption maxima and effect of solvents on absorption maxima of organic compounds

 

(In the end of the practical  the student should be able to)

Determine absorption and effect of solvent  on absorption maxima

  instrument demonstration /Practical demonstration

 

03

 

 

2.       Estimation of dextrose by colorimetry Determine the concentration of dextrose using colorimetry To calculate the dextrose by colorimetry method instrument demonstration /Practical demonstration

 

03

 

3.       Estimation of sulfanilamide by colorimetry

 

 Determine the concentration of sulfanilamide by colorimetry To calculate the sulfanilamide by colorimetry method instrument demonstration /Practical demonstration

 

03

 

4.       Simultaneous estimation of ibuprofen and paracetamol by UV spectroscopy

 

To plot calibration curve and estimate absorption and concentration of paracetamol and ibuprofen simultaneously To find out the comcentration of drugs in combination formulation. instrument demonstration /Practical demonstration

 

03

 

5.       Assay of paracetamol by UV- Spectrophotometry

 

determine absorption and concentration of paracetamol by  UV Spectrophotometer

 

To determine the concentration of PCM by UV method of analysis. instrument demonstration /Practical demonstration

 

03

 

6.       Estimation of quinine sulfate by fluorimetry

 

estimate quinine sulfate with the help of fluorimetry To find out the quinine sulfate  in the given mixture by fluorimetry method instrument demonstration /Practical demonstration

 

03

 

7.       Study of quenching of fluorescence

 

explain quenching  of fluorescence

 

  instrument demonstration /Practical demonstration

 

03

 

8.       Determination of sodium by flame photometry

 

determine sodium by flame  photometry

 

To find out the concentration of sodium by flame photometry instrument demonstration /Practical demonstration

 

03

 

9.       Determination of potassium by flame photometry

 

 

determine potassium by flame photometry

 

To find out the concentration of potassium by flame photometry instrument demonstration /Practical demonstration

 

03

 

10.    Determination of chlorides and sulphates by nephelo turbidometry

 

determine turbidity of chloride and sulphates by nephelo turbidometry

 

To find out the concentration of chlorides and sulphate by nephroturbidometry method of analysis instrument demonstration /Practical demonstration

 

03

 

11.    Separation of amino acids by paper chromatography

 

perform paper chromatography and separate different  amino acids

 

To separate the amino acid mixture by paper chromatography method. instrument demonstration /Practical demonstration

 

03

 

12.    Separation of sugars by thin layer chromatography

 

separate the sugars  by  TLC

 

To separate the sugar in the given mixture by TLC methods instrument demonstration /Practical demonstration

 

03

 

13.    Separation of plant pigments by column chromatography

 

Separate plants pigments by column chromatography

 

Separate the plant pigments in the given mixture by column chromatography method instrument demonstration /Practical demonstration

 

03

 

14.    Demonstration experiment on HPLC

 

separate components’ from the compounds  with the help of HPLC and determine retention time

 

To separate the different component of mixture using HPLC method of analysis. instrument demonstration /Practical demonstration

 

03

 

15.    Demonstration experiment on Gas Chromatography explain gas chromatography

 

Explain about the gas chromatography method of analysis. instrument demonstration /Practical demonstration

 

03