Curriculum | Biochemistry Practical | B. Pharmacy

Course: B. Pharmacy 2nd Semester/ 1st  Year

Name of Subject: Biochemistry Practical

Subject Code:  BP209P

S No.

Contents of the Topics

Learning objectives Teaching guidelines Methodology Time
(Hrs)
1 Qualitative analysis of carbohydrates (Glucose, Fructose, Lactose, Maltose, Sucrose and starch) (After doing the practical, the students should be able  to)

Perform qualitative tests for carbohydrates such as monosachharides, disachharides and polysachharides.
Able to handle all chemicals carefully

Benedict’s test,
Fehling’s test,
Molisch’s Test,
Barfoed’s Test,
Seliwanoff’s Test,
Iodine Test,
Bial’s Test
Instructive

Demonstrative

Actual performance by the students

06
2 Identification tests for Proteins (albumin and Casein) Perform qualitative test for proteins and amino acids Biuret test,
Xanthoproteic test,
Ninhydrin test,
Million’s test
Instructive

Demonstrative

Actual performance by the students

03
3 Quantitative analysis of reducing sugars (DNSA method) and Proteins (Biuret method) Perform quantitative estimation of proteins and reducing sugars
Familiar with various reagents, instruments, glassware’s and equipments required for understanding the practical.
Benedict’s test,
Fehling’s test
Instructive

Demonstrative

Actual performance by the students

06
4 Qualitative analysis of urine for abnormal constituents Understand the significance of abnormal constituents of urine Tests for Protein,
sugar,ketone bodies,blood,bile etc
Instructive

Demonstrative

Actual performance by the students

06
5 Determination of blood creatinine Understand the diagnostic application of blood creatinine BUN test Instructive

Demonstrative

Actual performance by the students

03
6 Determination of blood sugar Determine blood sugar level appropriately and apply that knowledge in understanding the significance of blood sugar level in Diabetic Conditions Using Glucometer Instructive

Demonstrative

Actual performance by the students

03
7 Determination of serum total cholesterol Determine serum total cholesterol level and understand the same in normal and pathological conditions LDL
HDL tests
Instructive

Demonstrative

Actual performance by the students

03
8 Preparation of buffer solution and measurement of pH Prepare and measure the pH of buffer solutions Measurement of ph of solutions using ph-meter Instructive

Demonstrative

Actual performance by the students

03
9 Study of enzymatic hydrolysis of starch Know the effect of enzymes on substrate concentrations Concept of hydrolysis and enzymatic hydrolysis of starch. Instructive

Demonstrative

Actual performance by the students

03
10 Determination of Salivary amylase activity Determine the activity of salivary amylase enzymes on hydrolysis of starch Salivary amylase activity Instructive

Demonstrative

Actual performance by the students

03
11 Study the effect of Temperature on Salivary amylase activity Study the effect of temperature on α-amylase activity and to determine the optimum temp. value by plotting graph Factors effecting the salivary amylase activity like temperature etc. Instructive

Demonstrative

Actual performance by the students

03
12 Study the effect of substrate concentration on salivary amylase activity Study the effect of substrate concentration on the activity of α-amylase enzyme Factors effecting the salivary amylase activity like substrate concentration etc. Instructive

Demonstrative

Actual performance by the students

03