Curriculum | ADVANCED INSTRUMENTATION TECHNIQUES | B. Pharmacy

Course: B Pharmacy 4th Year / 8th Semester

Name of Subject: ADVANCED INSTRUMENTATION TECHNIQUES

Subject Code: BP 811 ET.

Sr. No.

Topic (Experiments)

Learning objectives

Teaching Guidelines

Methodology

 
Nuclear Magnetic Resonance spectroscopy

Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin – spin coupling, relaxation, instrumentation and applications
Mass  Spectrometry-  Principles,  Fragmentation,  Ionization  techniques  –

Electron  impact,  chemical  ionization,   MALDI,  FAB,             Analyzers-Time  of
flight and Quadrupole, instrumentation, applications

(At the end of the session the student should be able to)

know about principle, Instrumentation, chemical shift, coupling constant and applications of Nuclear Magnetic Resonance spectroscopy

know about principle, Instrumentation, Fragmentation,  Ionization  techniques  and applications of Mass  Spectrometry

Concept of Nuclear Magnetic Resonance spectroscopy

Principles of H-NMR and C-NMR, chemical shift, factors affecting chemical shift, coupling constant, Spin – spin coupling, relaxation, instrumentation and applications
Mass  Spectrometry-  Principles,  Fragmentation,  Ionization  techniques  – Electron  impact,  chemical  ionization,   MALDI,  FAB,     Analyzers-Time  of
flight and Quadrupole, instrumentation, applications

Practical demonstration of working of Nuclear Magnetic Resonance spectroscopy

 

Instructive

Power Point Presentation

 
Thermal Methods of Analysis: Principles, instrumentation and applications
of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC)
X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X-ray
Crystallography, rotating crystal technique, single crystal diffraction ,powder
diffraction, structural elucidation and applications.
know about Principles, instrumentation and applications

of (TGA), (DTA), (DSC) and X-Ray Diffraction Methods

Study of thermal methods of analysis its principle instrumentation and applications
of ThermogravimetricAnalysis (TGA), Differential Thermal Analysis (DTA), Differential Scanning Calorimetry (DSC) X-Ray Diffraction Methods: Origin of X-rays, basic aspects of crystals, X-ray
Crystallography, rotating crystal technique, single crystal diffraction ,powder
diffraction, structural elucidation and applications.
 

Instructive

Power Point Presentation

Group discussion

 
Calibration and validation-as per ICH and USFDA guidelines
Calibration of following Instruments
Electronic  balance,  UV-Visible  spectrophotometer,  IR  spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC
Calibrate the instruments like Electronic  balance,  UV-Visible  spectrophotometer,  IR  spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC as per ICH and USFDA guidelines Explain Calibration and validation-as per ICH and USFDA guidelines and calibration of instruments i.e Electronic  balance,  UV-Visible  spectrophotometer,  IR  spectrophotometer, Fluorimeter, Flame Photometer, HPLC and GC Practical demonstration of working of instruments

 

Instructive

Power Point Presentation

 
Radio immune assay: Importance, various components, Principle, different

methods, Limitation and Applications of Radio immuno assay

Extraction techniques:General principle and procedure involved in the solid

phase extraction and liquid-liquid extraction

Know the Importance, various components, Principle, different

Methods and applications of Radio immuno assay

Familiar with the general principle and procedure involved in the solid phase extraction and liquid-liquid extraction

Concept of radio immune assay its importance,components,principle,methods
, Limitation and Applications of Radio immuno assay
Extraction techniques its principle, procedure and also phase extraction and liquid liquid extraction
Instructive

 

 

Power Point Presentation

 

Group discussion

 
Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS. Describe the Hyphenated techniques like LC-MS/MS, GC-MS/MS, HPTLC-MS etc. Concept of Hyphenated techniques-LC-MS/MS, GC-MS/MS, HPTLC-MS. Instructive

Power Point Presentation

Group discussion